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Vorbereitung

signalling by nuclear transport in plants Kircher et al. 1999

Nachbereitung
Vertiefung Master

Plant BioMEMs

What we expect you to know

Terminology

You should know, what these terms mean and you should be able to explain them in your own words.

second messengers . scalar . vectorial . genetic engineering . chemical engineering . enhancer trap . FACS . flow cytometry . laser ablation . laser-capture microdissection . aquaporins . microinjection . cell permeating peptides . peptoids . laser tweezer . caged compounds . optogenetics

Manipulation: Exercise

1. You want to test the model that the anterior-posterior polarity of Drosophila is produced by a gradient of the transcription factor bicoid. How can you show experimentally that a bicoid gradient is needed? What would happen, if you overexpress or knock down bicoid by genetic engineering?

2. How can the following molecules pass the plasma membrane barreer? water, glucose, NaCl, insulin, the membrane-penetrating peptoid CoQ, the natural auxin indole acetic acid?

3. You want to test, which genes are differentially expressed in the quiescent center (few cells in the central part of the root) compared to other epidermal cells. Describe an experimental strategy to get sufficient material for doing this experiment.

4.  You heard about an approach to engineer a light-switchable nuclear transport of a protein. Do you know natural proteins that are doing this already without engineering?