Constructs: Kinesins

For all constructs there are data sheets with details. Aliquots of the constructs can be obtained from Nadja Wunsch.

 

 Accession

 what is it

 use

data sheet 

       
    Rice KCH1 survey of tools for OsKCH1

K001

OsKCH1fl-Entry Entry vector full-length for GATEWAY cloning

pdf

K002 OsKCH1mot-Entry  Entry vector motor domain for GATEWAY cloning 

pdf

K003 OsKCH1ch-Entry  Entry vector CH domain for GATEWAY cloning 

pdf

K004 OsKCH1fl-His full length, recombinant expression pdf
K005 OsKCH1mot-His motor domain, recombinant expression pdf
K006 OsKCH1ch-His CH domain, recombinant expression pdf
       
    Rice DLK  
       
    GATEWAY entry constructs  
       
K021 pDONR-DLK (C) Entry vector full-length for GATEWAY cloning without stop codon (1-2292 bp)  
K022 pDONR-DLK (N) Entry vector full-length for GATEWAY cloning with stop codon (1-2295 bp)  
K023 pDONR-DLK-motor (C) Entry vector motor domain for GATEWAY cloning without stop codon (1111-2292 bp)  
K024 pDONR-DLK-motor (N) Entry vector motor domain for GATEWAY cloning with stop codon (1111-2295 bp)  
       
    GFP-fusions  
       
K025 pK7FWG2.0-DLK DLK full length (1-2292 bp) with GFP fused to its C-terminus, KanR  
K026 pK7WGF2.0-DLK DLK full length (1-2295 bp) with GFP fused to its N-terminus, KanR  
K027 pK7FWG2.0-DLK-motor DLK motor domain (1111-2292 bp) with GFP fused to its C-terminus, KanR  
K028 pK7WGF2.0-DLK-motor DLK motor domain (1111-2295 bp) with GFP fused to its N-terminus, HygR  
       
    RFP-fusions  
       
K025 pH7RWG2.0-DLK DLK full length (1-2292 bp) with RFP fused to its C-terminus, HygR  
K026 pH7WGR2.0-DLK DLK full length (1-2295 bp) with RFP fused to its N-terminus, KanR  
K027 pH7RWG2.0-DLK-motor DLK motor domain (1111-2292 bp) with RFP fused to its C-terminus, KanR  
K028 pH7WGR2.0-DLK-motor DLK motor domain (1111-2295 bp) with RFP fused to its N-terminus, HygR  
       
    recombinant expression  
       
K029 pDEST42-DLK-Tail DLK tail (1-1209bp) with His tag fused to its C-terminus