Constructs: Kinesins
For all constructs there are data sheets with details. Aliquots of the constructs can be obtained from Nadja Wunsch.
|
Accession |
what is it |
use |
data sheet |
| Rice KCH1 | survey of tools for OsKCH1 | ||
|
K001 |
OsKCH1fl-Entry | Entry vector full-length for GATEWAY cloning | |
| K002 | OsKCH1mot-Entry | Entry vector motor domain for GATEWAY cloning | |
| K003 | OsKCH1ch-Entry | Entry vector CH domain for GATEWAY cloning | |
| K004 | OsKCH1fl-His | full length, recombinant expression | |
| K005 | OsKCH1mot-His | motor domain, recombinant expression | |
| K006 | OsKCH1ch-His | CH domain, recombinant expression | |
| Rice DLK | |||
| GATEWAY entry constructs | |||
| K021 | pDONR-DLK (C) | Entry vector full-length for GATEWAY cloning without stop codon (1-2292 bp) | |
| K022 | pDONR-DLK (N) | Entry vector full-length for GATEWAY cloning with stop codon (1-2295 bp) | |
| K023 | pDONR-DLK-motor (C) | Entry vector motor domain for GATEWAY cloning without stop codon (1111-2292 bp) | |
| K024 | pDONR-DLK-motor (N) | Entry vector motor domain for GATEWAY cloning with stop codon (1111-2295 bp) | |
| GFP-fusions | |||
| K025 | pK7FWG2.0-DLK | DLK full length (1-2292 bp) with GFP fused to its C-terminus, KanR | |
| K026 | pK7WGF2.0-DLK | DLK full length (1-2295 bp) with GFP fused to its N-terminus, KanR | |
| K027 | pK7FWG2.0-DLK-motor | DLK motor domain (1111-2292 bp) with GFP fused to its C-terminus, KanR | |
| K028 | pK7WGF2.0-DLK-motor | DLK motor domain (1111-2295 bp) with GFP fused to its N-terminus, HygR | |
| RFP-fusions | |||
| K025 | pH7RWG2.0-DLK | DLK full length (1-2292 bp) with RFP fused to its C-terminus, HygR | |
| K026 | pH7WGR2.0-DLK | DLK full length (1-2295 bp) with RFP fused to its N-terminus, KanR | |
| K027 | pH7RWG2.0-DLK-motor | DLK motor domain (1111-2292 bp) with RFP fused to its C-terminus, KanR | |
| K028 | pH7WGR2.0-DLK-motor | DLK motor domain (1111-2295 bp) with RFP fused to its N-terminus, HygR | |
| recombinant expression | |||
| K029 | pDEST42-DLK-Tail | DLK tail (1-1209bp) with His tag fused to its C-terminus |