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KIT - Botanisches Institut II

Molekularbiologie und

Biochemie der Pflanzen



Prof. Dr. Holger Puchta


Botanisches Institut II

Gebäude 06.35

Hertzstr. 16

D-76187 Karlsruhe

Tel.: +49-721-608-43833

Fax: +49-721-608-44874


Die Molekularbiologie befindet sich an der Westhochschule (Hertzstraße 16, Geb. 06.35, 2. OG).

Die Biochemie befindet sich am FZU (Adenauerring 20 b, Geb. 50.40).

Willkommen am Botanischen Institut II

AG Puchta


Recker et al. (2014) Abb.
The Arabidopsis thaliana Homolog of the Helicase RTEL1 Plays Multiple Roles in Preserving Genome Stability

Julia Recker, Alexander Knoll, and Holger Puchta

The RTEL1 homolog in Arabidopsis thaliana plays multiple roles in preserving genome stability. RTEL1 suppresses homologous recombination in a pathway parallel to that of the DNA translocase FANCM.

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The CRISPR/Cas system can be used as nuclease for in planta gene targeting

Simon Schiml, Friedrich Fauser and Holger Puchta

The integration of a foreign gene at a pre-selected locus can be achieved by in planta gene targeting using the CRISPR/Cas system. Thus, these techniques should be useful for application in crop plants for gene stacking to improve elite cultivars.

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The nuclease FAN1 is involved in DNA crosslink repair in Arabidopsis thaliana independently of the nuclease MUS81

Natalie J. Herrmann, Alexander Knoll and Holger Puchta

A double mutant of the plant homologs of FAN1 and the endonuclease MUS81 resulted in greater sensitivity against crosslinks than in the respective single mutants. These results indicate that the two nucleases define two independent pathways of crosslink repair in plants.

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CRISPR/Cas Plasmids & Protocols

We are happy to share all of our CRISPR/Cas reagents with the plant community.

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