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KIT - Botanisches Institut II

Molekularbiologie und

Biochemie der Pflanzen



Prof. Dr. Holger Puchta


Botanisches Institut II

Gebäude 06.35

Hertzstr. 16

D-76187 Karlsruhe

Tel.: +49-721-608-43833

Fax: +49-721-608-44874


Die Molekularbiologie befindet sich an der Westhochschule (Hertzstraße 16, Geb. 06.35, 2. OG).

Die Biochemie befindet sich am FZU (Adenauerring 20 b, Geb. 50.40).

Willkommen am Botanischen Institut II

AG Puchta


Recker et al. (2014) Abb.
Current Publication

The RTEL1 homolog in Arabidopsis thaliana plays multiple roles in preserving genome stability. RTEL1 suppresses homologous recombination in a pathway parallel to that of the DNA translocase FANCM.

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Current Publication

The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny.

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Current Publication

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.

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CRISPR/Cas Plasmids & Protocols

We are happy to share all of our CRISPR/Cas reagents with the plant community.

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