Botanisches Institut

AG Puchta - Review Articles


Merker, L.; Schindele, P. & Puchta, H. (2020): Using CRISPR/ttLbCas12a for in planta gene targeting in A. thaliana. In: Current Protocols in Plant Biology, 5, e20117. DOI: 10.1002/cppb.20117

Schindele, P.; Wolter, F. & Puchta, H. (2020): CRISPR Guide RNA Design Guidelines for Efficient Genome Editing. In: Methods in molecular biology (Clifton, N.J.) 2166, S. 331–342. DOI: 10.1007/978-1-0716-0712-1_19

Khosravi, S.; Dreissig, S.; Schindele, P.; Wolter, F.; Puchta, H. & Houben, A. (2020): Live-Cell Imaging in Plant Cells with a Telomere-Specific Guide RNA. In: Methods in molecular biology (Clifton, N.J.) 2166, S. 343-356. DOI: 10.1007/978-1-0716-0712-1_20

Capdeville, N.; Schindele, P. & Puchta, H. (2020): Application of CRISPR/Cas-mediated base editing for directed protein evolution in plants. In: Science China. Life sciences. DOI: 10.1007/s11427-020-1655-9.

Hacker, L.; Dorn, A. & Puchta, H. (2020) Repair of DNA-protein crosslinks in plants. In: DNA Repair, 87 (2020) 102787. DOI:10.1016/j.dnarep.2020.102787

Dorn A. & Puchta H. (2020) Analyzing Somatic DNA Repair in Arabidopsis Meiotic Mutants. In: Pradillo M., Heckmann S. (eds) Plant Meiosis. Methods in Molecular Biology, vol 2061. Humana, New York, NY. DOI: 10.1007/978-1-4939-9818-0_25

Schindele, A.; Dorn, A. & Puchta, H. (2020): CRISPR/Cas brings plant biology and breeding into the fast lane. In: Current Opinion in Biotechnology 61, S. 7–14. DOI: 10.1016/j.copbio.2019.08.006



Dorn, Annika; Puchta, Holger (2019) DNA Helicases as Safekeepers of Genome Stability in Plants. Genes 2019, 10, 1028 DOI: 10.3390/genes10121028

Enderle, Janina; Dorn, Annika; Puchta, Holger (2019): DNA- and DNA-Protein-Crosslink Repair in Plants. In: International Journal of Molecular Sciences 20 (17). DOI: 10.3390/ijms20174304

Schmidt, Carla; Schindele, Patrick; Puchta, Holger (2019): From gene editing to genome engineering: restructuring plant chromosomes via CRISPR/Cas. In: aBIOTECH 36, S. 17. DOI: 10.1007/s42994-019-00002-0

Wolter, Felix; Schindele, Patrick; Puchta, Holger (2019): Plant breeding at the speed of light: the power of CRISPR/Cas to generate directed genetic diversity at multiple sites. In: BMC Plant Biol 19 (1), S. 557. DOI: 10.1186/s12870-019-1775-1

Huang, Teng-Kuei; Puchta, Holger (2019): CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination. In: Plant cell reports. DOI: 10.1007/s00299-019-02379-0

Schmidt C., Pacher M., Puchta H. (2019) DNA Break Repair in Plants and Its Application for Genome Engineering. In: Kumar S., Barone P., Smith M. (eds) Transgenic Plants. Methods in Molecular Biology, vol 1864. Humana Press, New York, NY. DOI: 10.1007/978-1-4939-8778-8_17



Kumlehn, J.; Pietralla, J.; Hensel, G.; Pacher, M.; Puchta, H. (2018): The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology. In: Journal of integrative plant biology. DOI: 10.1111/jipb.12734

Wolter, F.; Puchta, H. (2018): Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants. In: Methods in molecular biology (Clifton, N.J.) 1830, S. 23–40. DOI: 10.1007/978-1-4939-8657-6_2.

Schindele, P. , Wolter, F. and Puchta, H. (2018), Transforming plant biology and breeding with CRISPR/Cas9, Cas12 and Cas13. FEBS Lett. . doi:10.1002/1873-3468.13073

Wolter, F. and Puchta, H. (2018), The CRISPR/Cas revolution reaches the RNA world: Cas13, a new Swiss Army knife for plant biologists. Plant J. Accepted Author Manuscript. . doi:10.1111/tpj.13899



Wolter F. and Puchta H. (2017) Genome Engineering mit CRISPR/Cas– Revolution in der Pflanzenzüchtung BIOspektrum. DOI: 10.1007/s12268-017-0782-8

Pacher, M., & Puchta, H. (2017). From classical mutagenesis to nuclease‐based breeding–directing natural DNA repair for a natural end‐product. The Plant Journal. DOI:10.1111/tpj.13469

Wolter F. and Puchta H. (2017)  Knocking out consumer concerns and regulator’s rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals. Genome Biology  DOI:10.1186/s13059-017-1179-1

Puchta H. (2017) Applying CRISPR/Cas for genome engineering in plants: the best is yet to come. Current Opinion in Plant Biology 2017, 36:1–8

Scheben A., Wolter F., Batley J., Puchta H., and Edwards D. (2017) Towards CRISPR/Cas crops – bringing together genomics and genome editing New Phytologist DOI: 10.1111/nph.14702



Schiml S. and Puchta H. (2016) Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas. Plant Methods (2016) 12:8 doi: 10.1186/s13007-016-0103-0

Puchta H. (2016) Using CRISPR/Cas in three dimensions: towards synthetic plant genomes, transcriptomes and epigenomes.  The Plant Journal (2016). doi: 10.1111/tpj.13100

Puchta, H. (2016). Breaking DNA in plants: how I almost missed my personal breakthrough. Plant Biotechnology Journal (2016) 14, pp. 437–440 doi: 10.1111/pbi.12420

Puchta, H. (2016). Genome engineering using CRISPR/Cas: getting more versatile and more precise at the same time Genome Biology 2016, 17:51 doi:10.1186/s13059-016-0922-3

Steinert, J., Schiml, S., & Puchta, H. (2016). Homology-based double-strand break-induced genome engineering in plants. Plant Cell Reports, 1-10.



Puchta H. and Fauser F. (2015) Double-Strand Break Repair and Its Application to Genome Engineering in Plants.
In Zhang F., Puchta H. and Thomson J.G. (eds.) Advances in New Technology for Targeted Modification of Plant Genomes, Springer, 1- 20.



Fauser F. and Puchta H. (2014) Molekulare Chirurgie - Gezielte Genomveränderungen mithilfe von synthetischen Nucleasen - Ein Durchbruch für die moderne Pflanzenzüchtung. Naturwissenschaftliche Rundschau.

Schröpfer S., Knoll A., Trapp O. and Puchta H. (2014). DNA Repair and Recombination in Plants. Molecular Biology. S. H. Howell, Springer New York. 2: 51-93.

Knoll A., Fauser F. and Puchta H. (2014) DNA recombination in somatic plant cells: mechanisms and evolutionary consequences. Chromosome Res. 22: 191-201.

Knoll A., Schröpfer S. and Puchta H. (2014) The RTR complex as caretaker of genome stability and its unique meiotic function in plants. Front. Plant Sci. 5:33.

Puchta H. and Fauser F. (2014) Synthetic nucleases for genome engineering in plants: prospects for a bright future. Plant J 78, 727–741



Puchta H. and Fauser F. (2013) Gene Targeting in Plants: 25 years later. Int. J. Dev. Biol. 57: 629-637.



Puchta H. and Hohn B. (2012) In Planta Somatic Homologous Recombination Assay Revisited: A Successful and Versatile, but Delicate Tool. Plant Cell, 24: 4324–43

Mannuss A., Trapp O. and Puchta H. (2012) Gene regulation in response to DNA damage. Biochim Biophys Acta 1819, 154-165.



Trapp O., Seeliger K. and Puchta H. (2011) Homologs of breast cancer genes in plants. Frontiers in Plant Science 2: 1-17.

Knoll A. and Puchta H. (2011) The role of DNA helicases and their interaction partners in genome stability and meiotic recombination in plants. Journal of Experimental Botany. J. Ex. Bot, 62: No 5, 1565–1579.



Puchta H. and Hohn B. (2010) Breaking news: Plants mutate right on target. Proc. Natl. Acad. Sci. USA 107, 11657-11658.



Puchta H, Kobbe D, Wanieck K, Knoll A, Suer S, Focke M and Hartung F. (2009) Role of Human Disease Genes for the Maintenance of Genome Stability in Plants. In: Q.Y. Shu (ed.), Induced Plant Mutations in the Genomics Era. Food and Agriculture Organization of the United Nations, Rome, 2009, 129-132



Hartung F. and Puchta H. (2006) The RecQ-like gene family in plants. J. Plant Physiol. 160, 287-296.



Puchta H. and Hohn B. (2005) Green light for gene targeting in plants. Proc. Natl. Acad. Sci. USA 102, 11961-11962.

Puchta H. (2005) The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution. J. Ex. Bot., 56: No 409, 1- 14.



Hartung F. and Puchta H. (2004) What comparative genomics tells us about the evolution of the eukaryotic recombination machinery. Current Genomics 5, 109-121.



Hohn B. and Puchta H. (2003) Some like it sticky: gene targeting in rice. Trends in Plant Sci. 8, 51-53.

Puchta H. (2003) Towards the ideal GMP: Homologous recombination and marker gene excision. J. Plant Physiol. 160, 743-754.

Puchta H. (2003) Marker-free transgenic plants. Plant Cell Tissue Organ Cult. 74, 123-134.



Puchta H. (2002) Gene replacement by homologous recombination in plants. Plant Mol. Biol. 48, 173-182.



Hohn B., Levy A. and Puchta H. (2001) Elimination of selection markers from transgenic plants. Curr. Opin. Biotech. 12, 139-143.



Puchta H. (2000) Removing selectable marker genes: taking the shortcut. Trends in Plant Sci. 5, 273-274.



Hohn B. and Puchta H. (1999) Gene therapy in plants. Proc. Natl. Acad. Sci. USA 96, 8321-8323.

Puchta H. (1999) Doppelstrangbruchreparatur und Genomevolution bei Pflanzen. Biospektrum 5, 105-108.



Puchta H. (1998) Towards targeted transformation in plants. Trends in Plant Sci. 3, 77-78.



Puchta H. and Hohn B. (1996) From centiMorgans to basepairs: Homologous recombination in plants. Trends in Plant Sci. 1, 340-348.



Puchta H., Swoboda P. and Hohn B. (1994) Homologous recombination in plants. Experientia 50, 277-284

Puchta H. and Meyer P. (1994) Substrate specificity of plant recombinases determined in extrachromosomal recombination systems. In “Homologous recombination and gene silencing in plants”, J. Paszkowski Edt., Kluwer Academic Publishers, Dordrecht Niederlande, 123-155.



Puchta H., Ramm K. and Sänger H.L. (1991) Hop latent viroid (HLVd) and the worldwide distribution of latent viroids in vegetatively propagated plants. In “Proceedings Int. Workshop on Hop Virus Diseases”, A. Eppler Edt., German Phytomedical Society Series, Ulmer Verlag Stuttgart, 181-190.