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CRISPR/Cas Plasmids (S.thermophilus)

Gateway cloning plasmid set Streptococcus thermophilus
 

  • pDe-St1-CAS9 (Nuclease) - sequence information
    • Cas9 expression vector (Cas9 driven by the constitutive Ubi4-2 promotor from parsley)
    • Binary Gateway destination vector (contains ccdB gene!)
    • Bacterial selection: Spectinomycin (100 mg/l)
    • Plant selection: Kanamycin (30 mg/l)

 

  • pEn-St1-Chimera (sgRNA) - sequence information
    • sgRNA coding vector (sgRNA can easily be customized for any target site of interest; sgRNA is driven by the Arabidopsis U6-26 promoter)
    • Gateway entry vector
    • Bacterial selection: Ampicillin (100 mg/l)